Ns 1 antigen

Published customer image: Recombinant Dengue type 1 NS1 protein ( PIP047A) and recombinant Dengue type 4 NS1 ( Image caption: ELISAs showing humoral immune responses of mice immunized with D1NS1-HEK and D4NS1-HEK cells. a and b: Mouse antisera of D1NS1-HEK (red, panel a) and D4NS1-HEK (blue, panel b) immunized mouse groups (4 mice each) were analyzed on recombinant D1NS1 and recombinant D4NS1, respectively. c and d: Serotype cross-reactive humoral immune responses of D1NS1-HEK (red, panel c) and D4NS1-HEK (blue, panel d) mouse groups were analyzed on recombinant D4NS1 and recombinant D1NS1, respectively. Blue line (panel c) and red line (panel d) = pool of D4NS1-HEK and D1NS1-HEK mouse antisera, respectively, to enable direct comparison with the level of serotype-specific immune responses. e and f: Analysis of pooled mouse sera (red = D1NS1-HEK (e), blue = D4NS1-HEK (f)) on an unrelated hexa-His tagged protein (MUL_3720) as a control for humoral responses against the hexa-His tag. Black line in all panels = pool of mouse sera before immunization (pre-bleed) From: Rose N, Pinho-Nascimento CA, Ruggieri A, Favuzza P, Tamborrini M, Roth H, Baroni de Moraes MT, Matile H, Jänisch T, Pluschke G, Röltgen K. Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization. This image is from an open access article distributed under terms of a Recombinant dengue virus type 1 NS1 antigen is a purified preparation of the...

D engue is a mosquito-borne viral infection which can cause a severe flu-like illness. The infection spreads through the bite of infected Aedes mosquitoes (Ae. aegypti or Ae. albopictus). There are 4 Dengue virus strains (DEN 1, DEN2, DEN3, and DEN4) commonly associated with dengue infection. Among these, DEN 2 is more commonly associated with outbreaks. As there has been a sudden rise in the cases of Dengue infection these days, here is a quick guide to know the symptoms and to prevent transmission of the virus. This guide will also help to identify what and when to get tested if you suspect dengue-like symptoms. Signs & Symptoms Of Dengue The symptoms of dengue usually appear 4-10 days after being bitten by an infected mosquito. The most common symptoms associated with dengue fever may include: – Fever as high as 104℉ or 40℃ – Severe headache – Eye pain, typically behind the eyes (Retro-orbital pain) – Muscle, bone, or joint pain – Rash – Nausea and vomiting – Loose motions – Decreased appetite – Weakness – Swollen glands The fever can last for 5-7 days, but it may lose intensity around the 3rd to 7th day of illness. If the symptoms still persist with marked fatigue and generalized weakness, this could be an indication of the beginning of the critical phase (rapid decrease in the platelet count). It is important to know that in some cases people may not develop any symptoms or illness even after getting the virus. Still, it is advisable to consult a doctor or carefully a...

Background Early diagnosis of dengue virus (DENV) infection can improve clinical outcomes by ensuring close follow-up, initiating appropriate supportive therapies and raising awareness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1) has proven to be a useful biomarker for early diagnosis of dengue. A number of rapid diagnostic tests (RDTs) and enzyme-linked immunosorbent assays (ELISAs) targeting NS1 antigen (Ag) are now commercially available. Here we evaluated these tests using a well-characterized panel of clinical samples to determine their effectiveness for early diagnosis. Methodology/Principal Findings Retrospective samples from South America were used to evaluate the following tests: (i) “Dengue NS1 Ag STRIP” and (ii) “Platelia Dengue NS1 Ag ELISA” (Bio-Rad, France), (iii) “Dengue NS1 Detect Rapid Test (1 st Generation)” and (iv) “DENV Detect NS1 ELISA” (InBios International, United States), (v) “Panbio Dengue Early Rapid (1 st generation)” (vi) “Panbio Dengue Early ELISA (2 nd generation)” and (vii) “SD Bioline Dengue NS1 Ag Rapid Test” (Alere, United States). Overall, the sensitivity of the RDTs ranged from 71.9%–79.1% while the sensitivity of the ELISAs varied between 85.6–95.9%, using virus isolation as the reference method. Most tests had lower sensitivity for DENV-4 relative to the other three serotypes, were less sensitive in detecting secondary infections, and appeared to be most sensitive on Day 3–4 post symptom onset. The spec...

Dengue virus (DENV) infection is the most common mosquito-transmitted viral infection. DENV infection can cause mild dengue fever or severe dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS). Hemorrhage and vascular leakage are two characteristic symptoms of DHF/DSS. However, due to the limited understanding of dengue pathogenesis, no satisfactory therapies to treat nor vaccine to prevent dengue infection are available, and the mortality of DHF/DSS is still high. DENV nonstructural protein 1 (NS1), which can be secreted in patients’ sera, has been used as an early diagnostic marker for dengue infection for many years. However, the roles of NS1 in dengue-induced vascular leakage were described only recently. In this article, the pathogenic roles of DENV NS1 in hemorrhage and vascular leakage are reviewed, and the possibility of using NS1 as a therapeutic target and vaccine candidate is discussed. General information about dengue Dengue virus (DENV) is the most common mosquito-borne flavivirus and threatens people in tropic and subtropical areas. The World Health Organization estimates that more than 2.5 billion people representing over 40% of the world’s population are at risk of dengue infection [ Several hypotheses have been proposed to explain the pathogenesis of DHF/DSS [ DENV structure The DENV particle is approximately 500 Å in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins: capsid (C, 100 amino acids), pre...

The medical use of the NS1 antigen test can be defined to diagnose dengue infections and is effective to 1st day detection. Additionally, NS1 assay is useful for differential diagnostics in regards to NS1 is present in the serum of infected persons directly at the onset of clinical symptoms in primary dengue infection and produces a strong humoral response. It is detectable before the appearance of IgM antibodies. See also [ ] • • References [ ] • ^ a b www.selectscience.net. • ^ a b Debroy, Sumitra (September 16, 2010). The Times of India. • www.cdc.gov . Retrieved 2015-12-01. • Amorim JH, Alves RP, Boscardin SB, Ferreira LC (March 2014). "The dengue virus non-structural 1 protein: risks and benefits". Virus Research. 181: 53–60. • wwwnc.cdc.gov . Retrieved 2015-12-01. • Narayan R, Raja S, Kumar S, Sambasivam M, Jagadeesan R, Arunagiri K, Krishnasamy K, Palani G (July 2016). The Indian Journal of Medical Research. 144 (1): 128–133. Further reading [ ] • Peeling, Rosanna W.; Artsob, Harvey; Pelegrino, Jose Luis; Buchy, Philippe; Cardosa, Mary J.; Devi, Shamala; Enria, Delia A.; Farrar, Jeremy; Gubler, Duane J.; Guzman, Maria G.; Halstead, Scott B.; Hunsperger, Elizabeth; Kliks, Susie; Margolis, Harold S.; Nathanson, Carl M.; Nguyen, Vinh Chau; Rizzo, Nidia; Vázquez, Susana; Yoksan, Sutee (1 December 2010). Nature Reviews Microbiology. 8 (12 Suppl): S30–S37. • www.medscape.com. Medscape . Retrieved 26 July 2016. • Tricou, V.; Vu, H. T.; Quynh, N. V.; Nguyen, C. V.; Tran, H....