ELISA (Enzyme-Linked ImmunoSorbent Assay) is an immunologic technique used to detect the presence and concentration of an antigen or antibody in a sample. The power of an ELISA is based on the extreme specificity of the antigen-antibody interaction. ELISAs have wide-ranging applications, especially as medical diagnostic tools.

ELISA Technique Types Of ELISA. Competitive ELISA – Microtiter well which is antigen-coated is filled with the antigen-antibody mixture. Principle of ELISA. ELISA works on the principle that specific antibodies bind the target antigen and detect the. ELISA Procedure. ELISA is one of the easiest.

Although a positive test on any of the NS1 assays would increase the probability of dengue to above 90% in a patient, a negative result would only reduce this probability to 23.0–29.3%. In contrast, this probability of false negative diagnosis would be further reduced to 14.7% (95%CI 11.4–18.6) if SD Bioline NS1/IgM/IgG combo was negative.

Different types of ELISA. The four main types of ELISAs are direct, indirect, sandwich and competitive. All of these tests are commonly performed in 96 well plates, using the bottom of the wells as the solid surface to immobilize the antigen-antibody complexes. The differences between the four types – and their advantages and disadvantages.