Dengue igg

Dengue is an infectious disease caused by ‘dengue virus’ (DENV) which is a RNA virus belonging to the genus Flavivirus. The disease’s major complications high grade fever, hemorrhage and low platelets count (Thrombocytopenia). Four (4) Sero-types of the virus (DV 1, 2, 3 and 4) exist that are capable of causing the full spectrum of the disease. The virus is transmitted by the Aedes aegypti mosquitoes which bite during the day and lay eggs in artificial water containers. The dengue virus may transmit via blood products and blood transfusions. No vaccine is available and prevention depends on the vector (mosquito) control. Laboratory diagnosis of the dengue fever is based on the serological testing. In primary infection, IgM antibodies are produced on the 4th day and persist for 1-2 months. The IgG antibodies appear at the end of 2nd week (14th day) and provide lifelong immunity. In secondary infection, IgG antibodies are raised 1-2 days after the appearance of symptoms along with IgM which appear on 4th day. The rapid tests available detect the presence of IgG and IgM antibodies. PRINCIPLE: The immuno-chromatographic test device is coated with Dengue virus envelope proteins. When the sample (serum/plasma) containing anti Dengue IgG and IgM is placed in the sample well, it reacts with the envelope proteins and an antigen antibody complex is formed. This complex moves along the strip to produce colored lines. REQUIREMENTS: • Serum/Plasma • Test device or cassette • Sample dil...

High levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI). The anti-dengue EDI mAb was employed as a capturer and EDII and EDIII, which are mainly involved in the induction of neutralizing antibodies in patients, were fully available to bind to anti-dengue IgM or IgG in patients. A one-way automatic blood separation device prevented reverse migration of plasma and maximize the capture of anti-dengue antibodies at the test lines. A clinical evaluation in the field proved that the novel RDT (sensitivities of 96.5% and 96.7% for anti-dengue IgM and IgG) is more effective in detecting anti-dengue antibodies than two major commercial tests (sensitivities of 54.8% and 82% for SD BIOLINE; 50.4% and 75.3% for PanBio). The innovative format of RDT can be applied to other infectious viral diseases. Dengue is a mosquito-borne viral infection that causes a flu-like illness and occasionally develops into severe diseases, such as dengue hemorrhagic fever and dengue shock syndrome Aedes aegypti and A. al...

Early and accurate diagnosis of dengue fever is essential for appropriate patient management. The course of treatment for dengue includes a diet full of fruits, cereals, and juices. This diet helps to fulfill the mineral and vitamin requirement of the patient. As the initial • High fever and headache for a prolonged period. • Deep red rashes on your skin are an alarming sign that something is wrong. • Severe joint and muscle pain in adults. • A feeling of a broken bone is a major symptom of dengue fever. • A runny nose is an initial symptom seen in children. • Loss of appetite in children is a symptom of initial dengue phase. • A slight cough and gum bleeding. • Feeling lethargic and drowsy. • Skin Rashes Some of the rare symptoms of dengue include bleeding, low blood pressure and a severe reduction in platelet count. The diagnosis is followed up with a series of tests using molecular methods. TLC Blood Test Total leucocyte test is performed on a blood sample to measure the count of white blood cells and infection in your blood. The average cost of this test ranges from Rs. 40 to 250. Normal count of WBC ranges between 4000-11000 for all genders. All you need is to consult a good doctor and get your reports checked so that right medication is taken on time. Prothrombin Time Test Prothrombin time test This test is performed to measure the time taken by your plasma to form a blood clot. It is an outpatient procedure, generally performed at diagnostic labs. In case you don’t ...

Dengue is an infectious disease caused by ‘dengue virus’ (DENV) which is a RNA virus belonging to the genus Flavivirus. The disease’s major complications high grade fever, hemorrhage and low platelets count (Thrombocytopenia). Four (4) Sero-types of the virus (DV 1, 2, 3 and 4) exist that are capable of causing the full spectrum of the disease. The virus is transmitted by the Aedes aegypti mosquitoes which bite during the day and lay eggs in artificial water containers. The dengue virus may transmit via blood products and blood transfusions. No vaccine is available and prevention depends on the vector (mosquito) control. Laboratory diagnosis of the dengue fever is based on the serological testing. In primary infection, IgM antibodies are produced on the 4th day and persist for 1-2 months. The IgG antibodies appear at the end of 2nd week (14th day) and provide lifelong immunity. In secondary infection, IgG antibodies are raised 1-2 days after the appearance of symptoms along with IgM which appear on 4th day. The rapid tests available detect the presence of IgG and IgM antibodies. PRINCIPLE: The immuno-chromatographic test device is coated with Dengue virus envelope proteins. When the sample (serum/plasma) containing anti Dengue IgG and IgM is placed in the sample well, it reacts with the envelope proteins and an antigen antibody complex is formed. This complex moves along the strip to produce colored lines. REQUIREMENTS: • Serum/Plasma • Test device or cassette • Sample dil...

Early and accurate diagnosis of dengue fever is essential for appropriate patient management. The course of treatment for dengue includes a diet full of fruits, cereals, and juices. This diet helps to fulfill the mineral and vitamin requirement of the patient. As the initial • High fever and headache for a prolonged period. • Deep red rashes on your skin are an alarming sign that something is wrong. • Severe joint and muscle pain in adults. • A feeling of a broken bone is a major symptom of dengue fever. • A runny nose is an initial symptom seen in children. • Loss of appetite in children is a symptom of initial dengue phase. • A slight cough and gum bleeding. • Feeling lethargic and drowsy. • Skin Rashes Some of the rare symptoms of dengue include bleeding, low blood pressure and a severe reduction in platelet count. The diagnosis is followed up with a series of tests using molecular methods. TLC Blood Test Total leucocyte test is performed on a blood sample to measure the count of white blood cells and infection in your blood. The average cost of this test ranges from Rs. 40 to 250. Normal count of WBC ranges between 4000-11000 for all genders. All you need is to consult a good doctor and get your reports checked so that right medication is taken on time. Prothrombin Time Test Prothrombin time test This test is performed to measure the time taken by your plasma to form a blood clot. It is an outpatient procedure, generally performed at diagnostic labs. In case you don’t ...

High levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI). The anti-dengue EDI mAb was employed as a capturer and EDII and EDIII, which are mainly involved in the induction of neutralizing antibodies in patients, were fully available to bind to anti-dengue IgM or IgG in patients. A one-way automatic blood separation device prevented reverse migration of plasma and maximize the capture of anti-dengue antibodies at the test lines. A clinical evaluation in the field proved that the novel RDT (sensitivities of 96.5% and 96.7% for anti-dengue IgM and IgG) is more effective in detecting anti-dengue antibodies than two major commercial tests (sensitivities of 54.8% and 82% for SD BIOLINE; 50.4% and 75.3% for PanBio). The innovative format of RDT can be applied to other infectious viral diseases. Dengue is a mosquito-borne viral infection that causes a flu-like illness and occasionally develops into severe diseases, such as dengue hemorrhagic fever and dengue shock syndrome Aedes aegypti and A. al...

Dengue is an infectious disease caused by ‘dengue virus’ (DENV) which is a RNA virus belonging to the genus Flavivirus. The disease’s major complications high grade fever, hemorrhage and low platelets count (Thrombocytopenia). Four (4) Sero-types of the virus (DV 1, 2, 3 and 4) exist that are capable of causing the full spectrum of the disease. The virus is transmitted by the Aedes aegypti mosquitoes which bite during the day and lay eggs in artificial water containers. The dengue virus may transmit via blood products and blood transfusions. No vaccine is available and prevention depends on the vector (mosquito) control. Laboratory diagnosis of the dengue fever is based on the serological testing. In primary infection, IgM antibodies are produced on the 4th day and persist for 1-2 months. The IgG antibodies appear at the end of 2nd week (14th day) and provide lifelong immunity. In secondary infection, IgG antibodies are raised 1-2 days after the appearance of symptoms along with IgM which appear on 4th day. The rapid tests available detect the presence of IgG and IgM antibodies. PRINCIPLE: The immuno-chromatographic test device is coated with Dengue virus envelope proteins. When the sample (serum/plasma) containing anti Dengue IgG and IgM is placed in the sample well, it reacts with the envelope proteins and an antigen antibody complex is formed. This complex moves along the strip to produce colored lines. REQUIREMENTS: • Serum/Plasma • Test device or cassette • Sample dil...

Early and accurate diagnosis of dengue fever is essential for appropriate patient management. The course of treatment for dengue includes a diet full of fruits, cereals, and juices. This diet helps to fulfill the mineral and vitamin requirement of the patient. As the initial • High fever and headache for a prolonged period. • Deep red rashes on your skin are an alarming sign that something is wrong. • Severe joint and muscle pain in adults. • A feeling of a broken bone is a major symptom of dengue fever. • A runny nose is an initial symptom seen in children. • Loss of appetite in children is a symptom of initial dengue phase. • A slight cough and gum bleeding. • Feeling lethargic and drowsy. • Skin Rashes Some of the rare symptoms of dengue include bleeding, low blood pressure and a severe reduction in platelet count. The diagnosis is followed up with a series of tests using molecular methods. TLC Blood Test Total leucocyte test is performed on a blood sample to measure the count of white blood cells and infection in your blood. The average cost of this test ranges from Rs. 40 to 250. Normal count of WBC ranges between 4000-11000 for all genders. All you need is to consult a good doctor and get your reports checked so that right medication is taken on time. Prothrombin Time Test Prothrombin time test This test is performed to measure the time taken by your plasma to form a blood clot. It is an outpatient procedure, generally performed at diagnostic labs. In case you don’t ...

High levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI). The anti-dengue EDI mAb was employed as a capturer and EDII and EDIII, which are mainly involved in the induction of neutralizing antibodies in patients, were fully available to bind to anti-dengue IgM or IgG in patients. A one-way automatic blood separation device prevented reverse migration of plasma and maximize the capture of anti-dengue antibodies at the test lines. A clinical evaluation in the field proved that the novel RDT (sensitivities of 96.5% and 96.7% for anti-dengue IgM and IgG) is more effective in detecting anti-dengue antibodies than two major commercial tests (sensitivities of 54.8% and 82% for SD BIOLINE; 50.4% and 75.3% for PanBio). The innovative format of RDT can be applied to other infectious viral diseases. Dengue is a mosquito-borne viral infection that causes a flu-like illness and occasionally develops into severe diseases, such as dengue hemorrhagic fever and dengue shock syndrome Aedes aegypti and A. al...

Dengue is an infectious disease caused by ‘dengue virus’ (DENV) which is a RNA virus belonging to the genus Flavivirus. The disease’s major complications high grade fever, hemorrhage and low platelets count (Thrombocytopenia). Four (4) Sero-types of the virus (DV 1, 2, 3 and 4) exist that are capable of causing the full spectrum of the disease. The virus is transmitted by the Aedes aegypti mosquitoes which bite during the day and lay eggs in artificial water containers. The dengue virus may transmit via blood products and blood transfusions. No vaccine is available and prevention depends on the vector (mosquito) control. Laboratory diagnosis of the dengue fever is based on the serological testing. In primary infection, IgM antibodies are produced on the 4th day and persist for 1-2 months. The IgG antibodies appear at the end of 2nd week (14th day) and provide lifelong immunity. In secondary infection, IgG antibodies are raised 1-2 days after the appearance of symptoms along with IgM which appear on 4th day. The rapid tests available detect the presence of IgG and IgM antibodies. PRINCIPLE: The immuno-chromatographic test device is coated with Dengue virus envelope proteins. When the sample (serum/plasma) containing anti Dengue IgG and IgM is placed in the sample well, it reacts with the envelope proteins and an antigen antibody complex is formed. This complex moves along the strip to produce colored lines. REQUIREMENTS: • Serum/Plasma • Test device or cassette • Sample dil...