How to Prevent Mobile Phase Problems Low sensitivity and rising baselines, noise, or spikes on the chromatogram can often be attributed to the mobile phase. Contaminants in the mobile phase are especially troublesome in gradient elution. The baseline may rise, and spurious peaks can appear as the level of the contaminated component increases.

How HPLC detectors work. Most HPLC detectors work by converting a physiochemical property of an analyte into an electrical signal. In other words, a detector ‘sees’ a sample and sends signals at consecutive time points throughout the sample run. Signal intensity should correlate with the amount – either mass or concentration – of the.

A hemoglobin electrophoresis test is a blood test used to measure and identify the different types of hemoglobin in your bloodstream. Hemoglobin is the protein inside red blood cells.