PCR-restriction fragment length polymorphism (RFLP) assay is a cost-effective method for SNP genotyping and mutation detection, but the manual mining for restriction enzyme sites is challenging and cumbersome.

No, eukaryotic cells do not have restriction endonuclease because DNA molecules of eukaryotes are heavily methylated. All the restriction endonucleases have been isolated from various strain of bacteria.

The term “restriction endonuclease mapping” covers a number of related techniques used to identify specific restriction enzyme recognition sites on small DNA molecules. (One of these methods was presented in an earlier problem-solving test of this series [ 1 ].)