Histology

If you study the structure and function of cells, tissues, or organs, your research will likely involve histology. We have compiled helpful tips, tricks, and how-to guides written by researchers with hands-on experience in histology basics to help you get started or improve your histology skills. We take you through the various stages of sample prep and the must-have items to get the job done, and provide insight into the various stains for histology and when to use them. How to Get Started with Histology Basics If you’re new to the world of histology, or still getting up to speed, you’ve come to the right place. In this section, we’ll give you the knowledge to help get you on your way to producing fantastic histology results. Here, you’ll discover the history of histology, learn the pitfalls to avoid when performing your experiments, and even learn about the simple pleasures histology can provide. How to Prepare Your Histology Tissue Samples Histology tissue sample preparation involves multiple critical steps, from fixing and embedding to sectioning. Getting your sample preparation right is key to success with histology. Here we show you the different sample preparation techniques you can use, and top tips and tricks for becoming a master. Helpful Hints for When Histology Goes Wrong Whether you’re just beginning your journey or you’re a histology master, sometimes things just go wrong. If you find yourself with problems during sample prep or your stainings don't look righ...

This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.

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Eukaryotic cell Histology is the science of the microscopic structure of Key facts about histology Histology Microanatomy or microscopic anatomy, The study of cells and tissues, from their intracellular components to their organization into organs and Cell structure Cellular membrane, cytoplasm, organelles, nucleus Tissues A unity of cells with a similar structure that as a whole express a definite and unique function. Epithelial, connective, muscle, nervous Organs A unity of tissues with a more complex set of functions, defined by the combination of structure and function of the comprising tissues Systems of organs A group of organs united by similar functions. Cardiovascular, nervous, integumentary, musculoskeletal, respiratory, digestive, excretory, endocrine, lymphatic, reproductive Histology techniques Tissue preparation, tissue staining, microscopy, hybridisation + Show all Cells and tissues A nucleus and cytoplasm. The cytoplasm contains specialized subunits called Thoroughly learn the parts of a cell using our The DNA is condensed and coiled up into chromosomes. All cells are enveloped by a semipermeable two-layered membrane, which serves as a dynamic medium for the cell’s interaction with the external environment. Kind of like border police, it controls everything that comes in or out of the cell. Cells are categorized into various types, all of which perform different functions. These include epithelial cells, Cells and tissues Explore study unit Epithelial ti...

SecondLook™ Needs Your Support: Please support the SecondLook™ initiative with a donation to our SecondLook™ Donation Champaign (link is external). Your donation will enable us to update all existing SecondLook™ resources and to transform them into completely free Progressive Web Applications (PWA), including the SecondLook™Histology apps. It will also benefit the publication of several new topics (Hematology, Pathogen ID, and Gross Anatomy). Any size contribution is welcomed and will help us to provide these popular review tools to students at the University of Michigan and to many more worldwide. Resources Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources. • Be able to classify epithelial tissues. • Know the structure and function of junctions. • Know the structure of apical specializations and their functions. • Be able to correlate different types of epithelia to their functions. In epithelia, cells are organized in sheets, either a single layer thick (simple epithelia) or made up of multiple layers (stratified epithelia). Be able to identify the classes of epithelia underlined in the text below, and give some thought to why these different classes of epithelia ...