Reticulocytosis

To determine the adequacy of the regenerative response in dogs and cats, absolute reticulocyte counts can be calculated. This is the method used at Cornell University for determining the adequacy of a regenerative response as it takes into account the severity of the anemia but does not make assumptions about a “normal” hematocrit or correction factors for reticulocyte release. The Absolute reticulocyte count (thou/µL) = reticulocyte % (#/100) x RBC count (mill/µL) x 1000 or (even easier) Absolute reticulocyte count (thou/µL) = reticulocyte % x RBC count (mill/µL) x 10 Outlined below are approximate guidelines for assessing the degree or adequacy of regeneration in anemic dogs and cats, along with examples of using the results. Remember that cats have two different types of reticulocytes, aggregate and punctate. Only the aggregate reticulocytes are included in a reticulocyte count (as they are more reflective of current bone marrow production due to their short lifespan of <24 hours). ** The upper limit represents the he upper 95% confidence limit for our reference interval for absolute reticulocyte count in dogs and cats (i.e. some healthy animals have high counts, as expected for any interval). We can use the above table as a guideline for assessing the adequacy of the regenerative response, while taking into account the degree of anemia. For example, a dog with a severe anemia and a hematocrit (HCT) of 15% and a RBC count of 1.5 mill/µL has a reticulocyte count of 3%, w...

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The reticulocyte count is an extremely valuable blood test for determining the health of the bone marrow and can play a key role in the initial workup of anemia. Reticulocytes are "adolescent" red blood cells that have just been released from the bone marrow into the circulation and are present in the blood for only around one day before maturing into "adult" red blood cells. jarun011 / iStock The bone marrow is continually replenishing red blood cells so that roughly 1% of the red blood cells are reticulocytes at any time. A high reticulocyte count may be seen with bleeding or breakdown of red blood cells, as the bone marrow releases more reticulocytes to compensate for the loss. In contrast, a low reticulocyte count can mean that the bone marrow isn't functioning properly or that a deficiency (such as iron) is interfering with the production of red blood cells. • to evaluate abnormal findings on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platelet count. The count can also be helpful if levels of all types of blood cells are low ( • to evaluate low • to assess bone marrow function • to monitor treatment response after therapy is started for some types of anemia, such as that due to iron deficiency or vitamin B12 deficiency • to assess bone marrow function after chemotherapy • to assess the function of the bone marrow after a bone marrow transplant When the red blood cell count is low (when anemia is present), the bo...

Reticulocytosis Reticulocytosis: common although often the reticulocytes are destroyed by the antibody as well and reticulocytopenia may occur. From: Lanzkowsky's Manual of Pediatric Hematology and Oncology (Sixth Edition), 2016 Related terms: • Reticulocyte • Iron Deficiency • Erythropoietin • Spherocytosis • Haptoglobin • Thrombocytopenia • Erythropoiesis • Glucose-6-Phosphate Dehydrogenase • Hemoglobin Blood Level • Reticulocyte Count Faramarz Naeim, ... Wayne W. Grody, in Atlas of Hematopathology, 2013 Differential Diagnosis Spherocytosis, reticulocytosis, and positive antiglobulin (Coombs) tests are characteristic laboratory features of AIHA. AIHA is distinguished from hereditary spherocytosis by lack of a family history and positive antiglobulin tests. Alloantibody hemolytic anemia, sometimes observed in recipients of organ transplants, may mimic AIHA. Patients with PNH, similar to patients with CHAD, demonstrate hemoglobinuria. However, in PNH patients, flow cytometric studies for CD55 and CD59 show reduced or lack of expression of these antigens in blood cells, as well as positive acidified serum test and sucrose hemolysis test, whereas all these studies are negative in CHAD. 1. Anemia with reticulocytosis—moderate to severe in SS and Sβ 0-thalassemia, milder with SC or Sβ +-thalassemia. 2. Mean corpuscular volume (MCV) is normal with SS and microcytic with concomitant α-thalassemia; MCV is also microcytic with Sβ-thalassemia and SC genotypes. 3. Neutrophilia is co...

% RETICULOCYTES 1-3 days: 3.47-5.40% 4 days-4 weeks: 1.06-2.37% 5 weeks-7 weeks: 2.12-3.47% 8 weeks-5 months: 1.55-2.70% 6 months-23 months: 0.99-1.82% 24 months-5 years: 0.82-1.45% 6-11 years: 0.98-1.94% 12-17 years: 0.90-1.49% Adults: 0.60-2.71% ABSOLUTE RETICULOCYTES 1-3 days: 147.5-216.4 x 10(9)/L 4 days-4 weeks: 51.3-110.4 x 10(9)/L 5 weeks-7 weeks: 51.8-77.9 x 10(9)/L 8 weeks-5 months: 48.2-88.2 x 10(9)/L 6 months-23 months: 43.5-111.1 x 10(9)/L 24 months-5 years: 36.4-68.0 x 10(9)/L 6-11 years: 42.4-70.2 x 10(9)/L 12-17 years: 41.6-65.1 x 10(9)/L Adults: 30.4-110.9 x 10(9)/L 1. Adeli K, Raizman J, Chen Y, et al: Complex biological profile of hematologic markers across pediatric, adult, and geriatric ages: establishment of robust pediatric and adult reference intervals on the basis of the Canadian Health Measures Survey. Clin Chem 2015 Aug;61(8):1075-1086 2. CLSI. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline, Third Edition. CLSI document EP28-A3c. Wayne, PA, Clinical and Laboratory Standards Institute, 2008 3. Soldin J, Brugnara C, Wong EC: Pediatric Reference Intervals. Fifth edition. AACC Press. Washington DC 2005. ISBN 1-594250-32-4 4. Clinical Hematology: Principles, procedures, correlations. Second edition. Edited by CA Lotspeich-Steininger, EA Stiene-Martin, JA Koepke. Philadelphia, Lippincott-Raven, 1998, pp 114-117 The Sysmex XN 9000 reticulocyte analyzer is a flow cytometer that uses an argon laser ...