The iris microscope

General Sales Package • Pocket Microscope, Lab Manual, 10 Blank Nifty Slides, 3 Nifty Slides with specimens, Light Source, Water Resistant Pouch, Magic Tape (washable and re-usable), Notebook, Bookmark Skillset • Curiosity Building Number Of Batteries • 1 Batteries Minimum Age • 7 years Material • Plastic Battery Operated • Yes Battery Type • Lithium

• Adapters • • • • • Barlow Lens • Books & Experiments Cards • Bags & Cases • • • Cameras • Circuit Board Holders • Cleaning Kits • Condensers • • • • Dust Covers • Eye-Guards • Eyepieces • • • • • Filters • • • Fluorescence Kits • • • Focusing Racks • Fuses • Illuminators • • • • • • • • • Immersion Oils • Loupes • Magnifying Lamps • • • • Mechanical Stages • Monitors and Tablets • Calibration Slides & Stage Micrometers • Stage Warmers • Stain Kits • Stands • • • • Tweezers • Other Accessories Invented by a Dutch spectacle maker in the late 16th century, compound light microscopes use two sets of lenses to magnify images for study and observation. The first set of lenses are the oculars, or eyepieces, that the viewer looks into; the second set of lenses are the objectives, which are closest to the specimen. These lenses, along with a light source, allow scientists, technicians, and students to get an up-close view of samples. If this is your first time purchasing or using a microscope it’s a good idea to familiarize yourself with its construction to get the most out of this essential scientific instrument. Main Microscope Parts and Functions Head: The upper part of the microscope houses the eyepiece and objective lenses. Tube : Where the eyepieces are dropped in. Also, it connects the eyepieces to the objective lenses. Stage : The flat platform that supports the slides. Stage clips hold the slides in place. If your microscope has a mechanical stage, the slide is controlle...

Most microscopes provide a translation mechanism attached to the stage that allows the microscopist to accurately position, orient, and focus the specimen to optimize visualization and recording of images. The intensity of illumination and orientation of light pathways throughout the microscope can be controlled with strategically placed diaphragms, mirrors, prisms, beamsplitters, and other optical elements to achieve the desired degree of brightness and contrast in the specimen. Presented in Figure 1 is a typical microscope equipped with a trinocular head and 35-millimeter camera system for recording photomicrographs. Illumination is provided by a tungsten-halogen lamp positioned in the lamphouse, which emits light that first passes through a collector lens and then into an optical pathway in the microscope base. Also stationed in the microscope base is a series of filters that condition the light emitted by the incandescent lamp before it is reflected by a mirror and passed through the field diaphragm and into the substage condenser. The condenser forms a cone of illumination that bathes the specimen, located on the microscope stage, and subsequently enters the objective. Light leaving the objective is diverted by a beamsplitter/prism combination either into the eyepieces to form a virtual image, or straight through to the projection lens mounted in the trinocular extension tube, where it can then form a latent image on film housed in the camera system. The optical compo...

Posted in What is the diaphragm of a microscope, and how does it work? You’d have to say two things must perform exceptionally well if a microscope is going to function as it should. First of all, light must hit the object being viewed, and secondly, once the light has illuminated the specimen, it must be collected and magnified. One component essential to this procedure is the iris diaphragm. In this article, we’ll discuss what exactly the iris diaphragm is, how it works, and its responsibilities. What Is A Diaphragm? A diaphragm on a microscope is responsible for how much light leaves the To make this work, in the center of the diaphragm is a round hole called an aperture. The iris diaphragm controls the aperture size, which is where the light passes through. The diaphragm sits between the condenser and the specimen. The smaller the aperture on the iris diaphragm, the less light can pass through. In effect, this reduces the illumination of the specimen but increases the contrast. Moreover, there’s a direct comparison between the expansion and contraction of the microscope’s diaphragm, which is similar to that of the eye’s iris. What Is An Iris Diaphragm? The iris diaphragm is a design that allows adjustment and size of the aperture opening; as we previously mentioned, the name iris is taken from the eye. There are several types of iris diaphragms; the most popular and widely used ones utilize a set of blades in a circular arrangement. Controlling the blades affects the s...

Examination of the ultrastructure of the iris is essential for recognizing the structural specifications and differences in human irides. The vast range of structural differences represent themselves as a variety of different color eyes as it is seen in human populations. Here for the first time the ultrastructural comparison of light microscopy and electron microscopy of 3 color eyes (Blue, hazel/green and dark brown) has been described and the images presented side by side. These essential ultra-structure characteristics are identified and described as they play an important role in the reflected variety of iris structures and colors. There are multiple factors that are involved in the iris structural differences and it is the sum of all these factors, not an individual factor, that represent the final appearance of the iris color. Population of melanocytes on the anterior surface of the Iris. Population of melanosomes in the cytoplasm of melanocytes. Melanosomes location and distance from nucleus. Stages of maturation of melanosomes. The type of melanin and its ratio in the melanosomes. Population of fibroblasts on the iris surface. Amount of collagen in between and on the surface of the Iris. Number of Lysosomes in melanocytes. Number of cytoplasmic vacuoles in melanocytes. Number of mitochondria. Finger like projections in fibroblasts. Number of Stromal melanocytes. Number of Stromal macrophages. Stromal collagen content and intercellular matrix. An interesting observ...

Prepare, Light, Setup, Focus and Zoom. Five basic steps to get you jump started to becoming a microscope pro in no time. Once you have your specimen, and your light ready to go, you just setup your microscope and get focused. You can then iteratively increase the zoom power and get up close to your sample, and see the whole microscopic world! Eyepiece: The eyepiece lens on a microscope is the part of the optical system near where you place your eye to view the magnified object. In something like a pocket microscope, the eyepiece may not be adjustable or removable in any way, but in compound microscopes you can replace and upgrade them as you wish. There is so much to know about eyepieces we made a separate article on that topic called Objective: The objective lens on a microscope is part of the optical system that is responsible for producing a magnified image of the specimen being viewed. It is the lens closest to the specimen and is positioned at the bottom of the microscope’s nosepiece. There is a lot to talk about when it comes to objectives so we have a whole post dedicated to just that called Light Source / Illumination: Any microscope needs a light so you can see your object. This applies to a digital microscope to a pocket microscope to a compound microscope. There is different types of illumination sources, such as LED light microscopes to fluorescent microscope sources. In a pocket microscope, this can be as simple as a single LED, whereas expensive light sources...